Name:Recombinant TGF beta 1 (Transforming growth factor beta 1), Mouse, AF; Source:Mouse Cytokines; UniProt ID:P04202(Mouse), P17246(Rat); Host:Escherichia coli; Reactivity:Measure by its ability to inhibit the IL-4 dependent proliferation in HT-2 cells.
The ED50 for this effect is <0.1 ng/mL.; Application:Cell culture, Elisa; Calculated Mol Weight:14 kDa; Observed Mol Weight:16 kDa; Storage Condition:Lyophilized protein should be stored at -20°C for 1 year.
Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1%
BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months.; Recommended Dilution:It is recommended to reconstitute the lyophilized protein in sterile H2O to a
concentration not less than 100 μg/mL and incubate the stock solution for at least
20 min to ensure sufficient re-dissolved. In some experiments, it recommends to add 10 mM HCl when reconstitute lyophilized protein.; Buffer System:<0.1 EU per 1 μg of the protein by the LAL method. The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. 更多产品详情请至www.abways.com查询(按产品编号查询时请去除产品编号尾部的S)