别称 SKNDZ

组织来源 大脑

疾病 神经母细胞瘤细胞

传代比例/细胞消化 1:2传代,

完全培养基配置 DMEM培养基 ；10%胎牛血清 ；1%双抗

简介 SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma.SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma. Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells. Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression. Retinoic acid induces differentiation in this line.

形态 成神经细胞样

生长特征 贴壁生长

STR Amelogenin X CSF1PO 12 D3S1358 17 D5S818 12 D7S820 12,13 D8S1179 12,13 D13S317 8,11

倍增时间 每周 2 至 3 次

培养条件 气相：空气，95%；二氧化碳，5%。 温度：37°C，培养箱湿度为70%-80%。

冻存条件 冻存液：90%FBS，DMSO 10%,

保藏机构 ATCC; CRL-2149

备注 该细胞贴壁松散，收货如有大块脱落或全部脱落的细胞团，为正常现象，请按照收货注意事项处理。 【储存条件及期限】 T25瓶:观察好细胞状态后，75 %酒精消毒T25瓶外壁，将T25瓶置于37度培养箱放置2-4h，以便稳定细胞状态后传单；冻存管：收到细胞后，需立即转入液氮冻存或直接复苏