BFGF, a member of the fibroblast growth factor (FGF) family, is a growth factor and signaling protein encoded by the FGF2 gene. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. In normal tissue, bFGF is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. bFGF is a non-glycosylated, heparin-binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, monocytes, epithelial cells and endothelial cells. bFGF signals through FGFR 1b, 1c, 2c, 3c and 4. Thermostable FGF basic mutant was engineered for enhanced stability in culture media, without modification of its biological function, this thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule. Recombinant Human FGF-basic mutant G3 is a 17.3kDa protein consisting of 155 amino acid residues.
u Product Information
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Product Name
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Recombinant Human Basic Fibroblast Growth Factor mutant G3
(bFGF-G3)
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Synonyms
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Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 17.3kDa, a single non-glycosylated polypeptide chain containing155 amino acids.
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Accession
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P09038 Ala135-Ser288 with an N-terminal methionine and several site mutations
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AA Sequence
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MAAGSITTLPALPEDGGSGAFPPGHFKDPKLLYCKNGGFFLRIHPDGRVDGTRDKSDPFIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLYAIKNVTDECFFFERLEENNYNTYRSRKYPSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
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Purity
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＞97% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0 × 107 IU/mg.
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Formulation
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Lyophilized from 20mMTris,150mM NaCl, 5%trehalose,0.2‰Tween-20,pH7.6.
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Endotoxin level
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＜0.01EU/μg rHubFGF-G3 protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
3、 Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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【储存条件及期限】
冻干粉于-20℃到-80℃长期保存3年，溶液状态保存期3个月，避免反复冻融。
