Platelet-derived growth factors (PDGFs) are dimeric glycoprotein consisting of two 12.0-13.5 kDa polypeptide chains-chain A and chain B. The three naturally occurring subforms, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogen for cells of mesenchymal origin, including fibroblasts, smooth muscle cells and glial cells. PDGF is involved in various biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGFs signal through two receptors, PDGFR-α and PDGFR-β. Recombinant Human PDGF-BB is a 24.3kDa disulfide-linked homodimer of twoβchains, the βchain is a single non-glycosylated polypeptide chain containing 110 amino acids.
u Product Information
|
Product Name
|
Recombinant Human Platelet-derived Growth Factor-BB
（rHuPDGF-BB）
|
|
Source
|
Expressed in E. coli
|
|
Molecular weight
|
Approximately 24.8kDa, containing two 110 amino acid residues polypeptide
|
|
Accession
|
P01127 Ser82-Thr190 with an N-terminal Methionine
|
|
AA sequence
|
MSLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT
|
|
Purity
|
＞97% by SDS-PAGE or HPLC
|
|
Biological activity
|
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of > 3.3x105 IU/mg protein
|
|
Formulation
|
Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4
|
|
Endotoxin level
|
＜0.1EU/μg rHuPDGF-BB protein
|
|
Reconstitution
|
Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
3、 Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage/ Stability
|
For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
|
|
THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
|
【储存条件及期限】
冻干粉于-20℃到-80℃长期保存3年，溶液状态保存期3个月，避免反复冻融。
