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Product Name
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Recombinant Human soluble CD40 Ligand
(rHusCD40L)
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Synonyms
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soluble CD40 Ligand, TNFSF5, TRAP, CD154, Gp39, T-BAM
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 16.3kDa, a single non-glycosylated polypeptide chain containing 149 amino acids.
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Accession
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P29965 Met113-Leu261
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AA Sequence
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MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLWLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
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Purity
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＞97% by SDS-PAGE or HPLC
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Formulation
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Lyophilized from a 0.2mm filtered solution in in 20mM PB, 150mM NaCl, pH 7.0.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml.
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Endotoxin level
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Less than 1 EU/mg of rHusCD40L as determined by LAL method
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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White lyophilized powder. For long term storage, the product should be stored ≤-20℃. Please avoid repeated freeze-thaw cycles after reconstitution.
12 months from date of receipt, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
3 months, -20 to -70℃ under sterile conditions after reconstitution.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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