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Product Name
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Recombinant Rat ciliary neurotrophic factor
(rRtCNTF)
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Synonyms
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Ciliary neurotrophic factor;
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 22.7kDa, a single non-glycosylated polypeptide chain containing199 amino acids.
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Accession
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P20294 Ala2-Met200
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AA Sequence
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AFAEQTPLTLHRRDLCSRSIWLARKIRSDLTALMESYVKHQGLNKNINLDSVDGVPVASTDRWSEMTEAERLQENLQAYRTFQGMLTKLLEDQRVHFTPTEGDFHQAIHTLMLQVSAFAYQLEELMVLLEQKIPENEADGMPATVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRVISSHQMGISALESHYGAKDKQM
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Purity
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＞98% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg.
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Formulation
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Lyophilized from a 0.2µm filtered concentrated solution in 20mMPB, pH7.2, 300mM NaCl, 3% trehalose.
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Endotoxin level
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＜0.01EU/μg rRtCNTF protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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