Betacellulin (BTC) is a member of the EGF family of cytokines that also includes EGF, TGF-a, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulin. It is expressed in most tissues including kidney, uterus, liver and pancreas. BTC also presents in body fluids, including serum, milk, and colostrum. At the amino acid sequence level, human mature BTC protein exhibits 80% identity with mouse BTC protein. The protein binds to EGFR, ERBB4 and other EGF receptor family members and acts as a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. Recombinant Human Betacellulin is a 9.0kDa globular protein containing 80 amino acid residues，which comprises the mature EGF homologous portion of the Betacellulin protein.
u Product Information
|
Product Name
|
Recombinant Human Betacellulin
(rHuBetacellulin)
|
|
Synonyms
|
BTC
|
|
Source
|
Expressed in E. coli.
|
|
Molecular weight
|
Approximately 9.0kDa, a single non-glycosylated polypeptide chain containing 80 amino acids.
|
|
Accession
|
P35070 Asp32-Tyr111
|
|
AA Sequence
|
DGNSTRSPETNGLLCGDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKGRCRFVVAEQTPSCVCDEGYIGARCERVDLFY
|
|
Purity
|
＞96% by SDS-PAGE or HPLC.
|
|
Biological activity
|
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.05ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg..
|
|
Formulation
|
Lyophilized from a 0.2µm filtered concentrated solution in 20mMPB, 150mM NaCl, pH7.2.
|
|
Endotoxin level
|
＜0.01EU/μg rHuBetacellulin protein as determined by LAL method.
|
|
Reconstitution
|
Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage/ Stability
|
For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
|
|
THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
|
【储存条件及期限】
冻干粉于-20℃到-80℃长期保存3年，溶液状态保存期3个月，避免反复冻融。
