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Product Name
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Recombinant Human B-Cell Maturation Antigen/TNFRSF17
(rHuBCMA/TNFRSF17)
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Synonyms
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TNFRSF17, BCM, CD269
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 5.4kDa, a single non-glycosylated polypeptide chain containing 50 amino acids.
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Accession
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Q02223 Ala5- Ala54
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AA Sequence
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AGQCSQNEYFDSLLHACIPCQLRCSSNTPPLTCQRYCNASVTNSVKGTNA
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Purity
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＞98% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated murine B cells is no less than 40ng/ml, corresponding to a specific activity of >2.5×104 IU/mg in the presence of 100ng/ml human APRIL.
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Formulation
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Lyophilized from a 0.2μm filtered concentrated solution in 30% acetonitrile, 0.1% TFA.
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Endotoxin level
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＜0.1EU/μg rHuBCMA/TNFRSF17 protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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