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Product Name
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Recombinant Human Prolactin
(rHuProlactin)
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Synonyms
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Mammotropin, Luteotropic hormone, Luteotropin
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 23.0kDa, a single non-glycosylated polypeptide chain containing 200 amino acids.
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Accession
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P01236 Leu29-Cys227 with an N-terminal methionine
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AA Sequence
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MLPICPGGAARCQVTLRDLFDRAVVLSHYIHNLSSEMFSEFDKRYTHGRGFITKAINSCHTSSLATPEDKEQAQQMNQKDFLSLIVSILRSWNEPLYHLVTEVRGMQEAPEAILSKAVEIEEQTKRLLEGMELIVSQVHPETKENEIYPVWSGLPSLQMADEESRLSAYYNLLHCLRRDSHKIDNYLKLLKCRIIHNNNC
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Purity
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＞98% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. Determined by its ability to induce the proliferation of rat Nb2-11 cells in the concentration range of 0.1-1.0 ng/ml..
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Formulation
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Lyophilized from a 0.2µm filtered concentrated solution in PBS
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Endotoxin level
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＜0.01EU/μg rHuProlactin protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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White lyophilized powder. For long term storage, the product should be stored ≤-20℃
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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