Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced primarily by mast cells, Th2 cells, eosinophils and basophils. IL-4 is a key regulator in humoral and adaptive immunity and has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It regulates the differentiation of naive CD4+ T cells into helper Th2 cells and immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells is associated with allergies. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities. IL-4 has a compact, globular fold, stabilized by 3 disulfide bonds. The recombinant Interleukin-4 is a 15kDa protein consisting of 129amino acid residues.
u Product Information
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Product Name
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Recombinant Human Interleukin-4
(rHuIL-4)
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Synonyms
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BCGF, BCDF, B cell stimulating factor (BSF-1)
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Source
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Expressed in E. coli
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Molecular weight
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Approximately 15.0 kDa, 129 amino acids
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Accession
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P05112 His25-Ser153
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Protein amino acid sequence
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HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS
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Purity
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＞98% by SDS-PAGE or HPLC
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.
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Formulation
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Lyophilized from a 0.2μm filtered solution in 20mM PB, 150mM NaCl, 0.02% Tween-20, pH 5.0.
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Endotoxin level
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＜0.1EU/μg rHuIL-4 protein
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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【储存条件及期限】
冻干粉于-20℃到-80℃长期保存3年，溶液状态保存期3个月，避免反复冻融。
