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Product Name
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Recombinant Human Granulocyte Colony-stimulating Factor
(rHuG-CSF)
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Source
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Expressed in E. coli
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Molecular weight
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Approximately 18.7kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.
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Accession
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P09919 Thr31-Pro207
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AA Sequence
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TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP
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Purity
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＞97% by SDS-PAGE gel or HPLC
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×107 IU/mg.
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Formulation
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Lyophilized from a 0.2mm filtered solution in 10mM sodium acetate buffer, containing 3% trehalose, pH 4.0.
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Endotoxin level
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＜0.1EU/μg rHuG-CSF protein
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
3、 Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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