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Product Name
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Recombinant Human Stem Cell Factor
(SCF, Hematopoietic growth factor KL, MGF)
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Source
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Expressed in E. coli
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Molecular weight
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Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 164 amino acids.
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Accession
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P21583 Glu26-Ala189
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AA sequence
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EGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVA
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Purity
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＞98% by SDS-PAGE gel or HPLC
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is≤2.0ng/ml, corresponding to a specific activity of ≥5x 105 units/mg
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Formulation
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Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4
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Endotoxin level
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＜0.1EU/μg recombinant SCF protein
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
3、 Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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