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Product Name
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Recombinant Human Interleukin-17A
(rHuIL-17A)
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Synonyms
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IL-17, CTLA-8
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 31kDa, a homodimer of two 132 amino acid polypeptide chains.
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Accession
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Q16552 Gly24-Ala154 with a N-terminal Met
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AA Sequence
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MGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA
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Purity
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＞95% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 7.5ng/ml, corresponding to a specific activity of >1.3 ×105 IU/mg.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 20mM PB, 150mM NaCl, pH7.4
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Endotoxin level
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＜0.01EU/μg rHuIL-17A protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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White lyophilized powder. For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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