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Product Name
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Recombinant Human Interleukin-8, 77a.a./CXCL8
(rHuIL-8, 77a.a./CXCL8)
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Synonyms
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Emoctakin, GCP-1, MDNCF, MONAP, NAP-1, T-cell chemotactic factor, Protein 3-10C
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 8.9kDa, a single polypeptide chain containing 77 amino acids.
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Accession
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P10145 Ala23- Ser99
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AA Sequence
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AVLPRSAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKFLKRAENS
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Purity
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＞96% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human CXCR2 transfected mouse BaF3 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105IU/mg
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 20mM PB, 150mM NaCl, pH7.4
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Endotoxin level
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＜0.01EU/μg rHuIL-8,77a.a./CXCL8 protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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White lyophilized powder. For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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