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Product Name
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Recombinant Human Interleukin-9
(rHuIL-9)
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Synonyms
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p40 cytokine, T-cell growth factor p40
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 14.1kDa, a single non-glycosylated polypeptide chain containing126 amino acids.
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Accession
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P15248 Gln19- Ile144
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AA Sequence
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QGCPTLAGILDINFLINKMQEDPASKCHCSANVTSCLCLGIPSDNCTRPCFSERLSQMTNTTMQTRYPLIFSRVKKSVEVLKNNKCPYFSCEQPCNQTTAGNALTFLKSLLEIFQKEKMRGMRGKI
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Purity
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＞97% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human MO7e cells is less than 0.2ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 20mM PB, 150mM NaCl, pH7.4
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Endotoxin level
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＜0.01EU/μg rHuIL-9 protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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White lyophilized powder. For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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