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Product Name
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Recombinant Human Interleukin-31
(rHuIL-31)
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Synonyms
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IL31, IL-31, Interleukin31
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 15.8kDa, a single non-glycosylated polypeptide chain containing 141 amino acid
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Accession
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Q6EBC2 Ser24-Thr164
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AA Sequence
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SHTLPVRLLRPSDDVQKIVEELQSLSKMLLKDVEEEKGVLVSQNYTLPCLSPDAQPPNNIHSPAIRAYLKTIRQLDNKSVIDEIIEHLDKLIFQDAPETNISVPTDTHECKRFILTISQQFSECMDLALKSLTSGAQQATT
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Purity
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＞97% by SDS-PAGE or HPLC
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Formulation
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Lyophilized from a 0.2mm filtered solution in 20mM PB, 150mM NaCl, pH 7.4.
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Biological activity
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Fully biologically active when compared to standard. The specific activity is determined by inducing STAT3 activation using human U-87 MG cells. 5ng/mL of rHuIL-31 can effectively induce STAT3 activation.
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Endotoxin level
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＜0.01EU/μg rHuIL-31 protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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