Human IL-31 gene is located on Chr.12. It expresses the IL-31 protein at low levels in the type 2 helper T cells, which exits in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. This protein shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. IL-31 signals through IL-31 receptor A and Oncostatin M receptor subunits and can activate STAT3 through receptors and maybe involve in skin immunity. It regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. Human IL-31 shares only 24% a.a. sequence identity in the mature protein with mouse IL-31. Recombinant IL-31 is a single non-glycosylated polypeptide chain containing 141 amino acids
u Product Information
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Product Name
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Recombinant Human Interleukin-31
(rHuIL-31)
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Synonyms
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IL31, IL-31, Interleukin31
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 15.8kDa, a single non-glycosylated polypeptide chain containing 141 amino acid
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Accession
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Q6EBC2 Ser24-Thr164
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AA Sequence
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SHTLPVRLLRPSDDVQKIVEELQSLSKMLLKDVEEEKGVLVSQNYTLPCLSPDAQPPNNIHSPAIRAYLKTIRQLDNKSVIDEIIEHLDKLIFQDAPETNISVPTDTHECKRFILTISQQFSECMDLALKSLTSGAQQATT
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Purity
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＞97% by SDS-PAGE or HPLC
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Formulation
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Lyophilized from a 0.2mm filtered solution in 20mM PB, 150mM NaCl, pH 7.4.
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Biological activity
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Fully biologically active when compared to standard. The specific activity is determined by inducing STAT3 activation using human U-87 MG cells. 5ng/mL of rHuIL-31 can effectively induce STAT3 activation.
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Endotoxin level
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＜0.01EU/μg rHuIL-31 protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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【储存条件及期限】
冻干粉于-20℃到-80℃长期保存3年，溶液状态保存期3个月，避免反复冻融。
