PDGFs are disulfide-linked dimers consisting of two 12.0-13.5kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules, and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is a high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant Human PDGF-AA is a 28.8kDa disulfide-linked homodimer of two A chains (252 total amino acids).
u Product Information
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Product Name
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Recombinant Human Platelet-derived Growth Factor-AA
（rHuPDGF-AA）
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Synonyms
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Platelet-Derived Growth Factor-AA, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)
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Source
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Expressed in E. coli
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Molecular weight
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Approximately 28.8kDa, a disulfide-linked homodimeric protein containing two 126 amino acid residues polypeptide. It migrates with an apparent molecular mass of 33.6kDa.
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Accession
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P04085 Ser87-Thr211 with an N-terminal Methionine
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AA sequence
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MSIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT
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Purity
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＞98% by SDS-PAGE or HPLC
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Biological activity
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Fully biologically active when compared to standard. Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 50- 200ng/mL in a fluorometric assay using the redox sensitive dye.
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Formulation
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Lyophilized from a 0.2mm filtered solution in 20mM PB, 300mM NaCl, pH6.0
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Endotoxin level
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＜0.01EU/μg rHuPDGF-AA protein
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
3、 Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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【储存条件及期限】
冻干粉于-20℃到-80℃长期保存3年，溶液状态保存期3个月，避免反复冻融。
