|
Product Name
|
Recombinant Murine Fibroblast Growth Factor 21
(rMuFGF-21)
|
|
Synonyms
|
Fibroblast Growth Factor-21, FGFL
|
|
Source
|
Expressed in E. coli.
|
|
Molecular weight
|
Approximately 19.9kDa, a single polypeptide chain containing 182 amino acids.
|
|
Accession
|
Q9JJN1 Ala29-Ser210
|
|
AA Sequence
|
AYPIPDSSPLLQFGGQVRQRYLYTDDDQDTEAHLEIREDGTVVGAAHRSPESLLELKALKPGVIQILGVKASRFLCQQPDGALYGSPHFDPEACSFRELLLEDGYNVYQSEAHGLPLRLPQKDSPNQDATSWGPVRFLPMPGLLHEPQDQAGFLPPEPPDVGSSDPLSMVEPLQGRSPSYAS
|
|
Purity
|
＞96% by SDS-PAGE or HPLC.
|
|
Biological activity
|
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of >2.0×103 IU/mg in the presence of 5µg/ml of rMuβ-Klotho and 10μg/ml of heparin.
|
|
Formulation
|
Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB 500mM NaCl, pH7.4.
|
|
Endotoxin level
|
＜0.01EU/μg rMuFGF-21 protein as determined by LAL method.
|
|
Reconstitution
|
Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in a sterile aqueous buffer to an appropriate concentration.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage/ Stability
|
White lyophilized powder. For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
|
|
THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
|
