Product Name

Recombinant human Platelet Factor-4/CXCL4

(rHuPF-4/CXCL4)

Synonyms

Iroplact, Oncostatin-A

Source

Expressed in E. coli.

Molecular weight

Approximately 7.8kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

Accession

P02776 Ala32-Asn101

AA Sequence

EAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLES

Purity

＞97% by SDS-PAGE or HPLC.

Biological activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human fibroblasts is in a concentration of 1.0-10 ng/ml.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in Acetonitrile and TFA.

Endotoxin level

＜0.1EU/μg rHuPF-4/CXCL4 protein as determined by LAL method.

Reconstitution

Before use this product, please read the direction below carefully.

1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. 

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Storage/ Stability

White lyophilized powder. For long term storage, the product should be stored ≤-20℃. 

36 months, -20 to -70℃ as supplied.

1 month, 2 to 8℃ under sterile conditions after reconstitution;

3 months, -20 to -70℃ under sterile conditions after reconstitution.

Please avoid repeated freeze-thaw cycles.

THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.