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Product Name
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Recombinant Murine Interleukin-1 beta
(rMuIL-1β)
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Synonyms
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Catabolin, Leukocyte Endogenous Mediator, LEM, Lymphocyte-activating factor, LAF, Mononuclear Cell Factor, MCF, Endogenous Pyrogen, EP
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 17.5kDa, a single non-glycosylated polypeptide chain containing152 amino acids.
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Accession
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P10749 Val118-Ser269
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AA Sequence
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VPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS
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Purity
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＞96% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2pg/ml, corresponding to a specific activity of > 5.0×108IU/mg.
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Formulation
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Lyophilized from a 0.2µm filtered solution in 20mM PB, 150mM NaCl, pH7.4.
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Endotoxin level
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＜0.1EU/μg rMuIL-1βprotein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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