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Product Name
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Recombinant Murine Interleukin-6
(rMuIL-6)
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Synonyms
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B-cell hybridoma growth factor, Interleukin HP-1.
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Molecular weight
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Approximately 21.7kDa, a single polypeptide chain containing188 amino acids.
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Accession
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P08505 Phe25-Thr211, with an N-terminal Met
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AA Sequence
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MFPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELCNGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKARVLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQT
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Purity
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＞96% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is less than 0.02ng/ml, corresponding to a specific activity of >5 × 107 IU/mg.
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Formulation
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Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, 150mM NaCl, pH 7.0.
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Endotoxin level
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＜0.1EU/μg rMuIL-6 protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in in 100mM Acetic Acid to a concentration of 0.1mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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White lyophilized powder. For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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