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Product Name
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Recombinant murine Interferon-gamma
(rMuIFN-γ)
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 15.5kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.
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Accession
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P01580 His23-Cys155
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AA Sequence
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HGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQIISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQLLPESSLRKRKRSRC
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Purity
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＞97% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.8ng/ml, corresponding to a specific activity of >1.3×106IU/mg
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Formulation
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Lyophilized from a 0.2µm filtered concentrated solution in 20 mM Tris-HCl pH8.0, 300 mM NaCl, containing 5% trehalose, 0.05% Tween-20.
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Endotoxin level
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＜0.1EU/μg rMuIFN-γprotein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in a sterile aqueous buffer 20 mM Tris-HCl pH8.0 with 50 mM NaCl to a concentration of 0.1-0.3 mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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White lyophilized powder. For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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