|
Product Name
|
Recombinant human Cardiotrophin-1
(rHuCT-1)
|
|
Synonyms
|
CT-1
|
|
Source
|
Expressed in E. coli.
|
|
Molecular weight
|
Approximately 21.2kDa, a single non-glycosylated polypeptide chain containing 201 amino acids.
|
|
Accession
|
Q16619 Met1- Phe201 with an N-terminal methionine
|
|
AA Sequence
|
MSRREGSLEDPQTDSSVSLLPHLEAKIRQTHSLAHLLTKYAEQLLQEYVQLQGDPFGLPSFSPPRLPVAGLSAPAPSHAGLPVHERLRLDAAALAALPPLLDAVCRRQAELNPRAPRLLRRLEDAARQARALGAAVEALLAALGAANRGPRAEPPAATASAASATGVFPAKVLGLRVCGLYREWLSRTEGDLGQLLPGGSA
|
|
Purity
|
＞95% by SDS-PAGE or HPLC.
|
|
Biological activity
|
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1.0ng/ml, corresponding to a specific activity of > 1.0×106IU/mg.
|
|
Formulation
|
Lyophilized from a 0.2µm filtered concentrated solution in 20 mM PB, 150mM NaCl, 0.1% Tween-20 and 3% Trehalose, pH 7.4.
|
|
Endotoxin level
|
＜0.01EU/μg rHuCT-1 protein as determined by LAL method.
|
|
Reconstitution
|
Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage/ Stability
|
White lyophilized powder. For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
|
|
THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
|
