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Product Name
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Recombinant Human Neuregulin1-beta2
(rHuNRG1-β2)
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Synonyms
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HRG1-β2, Breast cancer cell differentiation factor p45, glial growth factor
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 7.0kDa, a single non-glycosylated polypeptide chain containing 61 amino acids.
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Accession
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Q02297 Ser177-Gln237
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AA Sequence
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SHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCPNEFTGDRCQNYVMASFYKAEELYQ
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Purity
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＞97% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 5ng/ml, corresponding to a specific activity of >2.0 ×105IU/mg.
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Formulation
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Lyophilized from a 0.2µm filtered concentrated solution in 20mMPB,150mM NaCl, pH7.4.
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Endotoxin level
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＜0.01EU/μg rHuNRG1-β2 protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃.
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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