Tetanus toxin is a potent neurotoxin secreted by the anaerobic bacterium Clostridium tetani and is the compound responsible for the symptoms of tetanus. The toxin consists of two polypeptide chains connected by a disulfide linkage. The heavy chain contains two regions of interest: a binding domain and a translocation domain. The binding domain allows the toxin to be taken up by neurons upon binding to gangliosides on nerve endings. Upon reaching inhibitory neurons via axonal and transsynaptic transport, the translocation domain allows the toxin to enter the cytosol. The light chain is a zinc endopeptidase, activated by enzymes in the cytosol that cleaves the disulfide bond between the heavy and light chain. Once activated, the light chain cleaves synaptobrevin 2, blocking release of inhibitory transmitters GABA and glycine to motor neurons, causing the prolonged muscle transactions symptomatic of tetanus. Enzymatic cleavage of tetanus toxin by papain generates two main molecules: 1)the light chain linked to the N-terminal portion of the heavy chain containing the translocation domain, and 2) the C-fragment, or the C-terminal portion of the heavy chain, which contains the binding domain. C-fragment is considered nontoxic and, when recombinantly produced, contains no trace of intact toxin.
In vitro, tetanus toxin C-fragment (TTC)is an efficient mucosal adjuvant and carrier molecule for the generation of mucosal antibody responses and/or induction of systemic T-cell tolerance to linked antigens. TTC is also used for tract tracing in neurological research, taking advantage of its high affinity to GT1B gangliosides and retrograde transport. Recombinant tetanus toxin C-fragment is a 51.6kDa globular protein containing 451 amino acids.
u Product Information
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Product Name
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Recombinant Tetanus toxin C Fragment
(rTTc)
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Source
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Expressed in E. coli
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Molecular weight
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Approximately 51.6kDa, a single non-glycosylated polypeptide chain containing 451 amino acids
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KNLDCWVDNEEDIDVILKKSTILNLDINNDIISDISGFNSSVITYPDAQLVPGINGKAIHLVNNESSEVIVHKAMDIEYNDMFNNFTVSFWLRVPKVSASHLEQYGTNEYSIISSMKKHSLSIGSGWSVSLKGNNLIWTLKDSAGEVRQITFRDLPDKFNAYLANKWVFITITNDRLSSANLYINGVLMGSAEITGLGAIREDNNITLKLDRCNNNNQYVSIDKFRIFCKALNPKEIEKLYTSYLSITFLRDFWGNPLRYDTEYYLIPVASSSKDVQLKNITDYMYLTNAPSYTNGKLNIYYRRLYNGLKFIIKRYTPNNEIDSFVKSGDFIKLYVSYNNNEHIVGYPKDGNAFNNLDRILRVGYNAPGIPLYKKMEAVKLRDLKTYSVQLKLYDDKNASLGLVGTHNGQIGNDPNRDILIASNWYFNHLKDKILGCDWYFVPTDEGWTND
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Purity
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＞96% by SDS-PAGE or HPLC，Dimers and high molecular weight impurities: <5% by SEC-HPLC
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Formulation
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Lyophilized from a 0.2mm filtered concentrated solution in PBS pH7.4.
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Biological activity
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Fully biologically active when compared to standard. Measured in a hemagglutination assay using sheep red blood cells coated with GT1B ganglioside, and exhibits comparable binding activity as standard.
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Endotoxin level
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Less than 0.01EU/mg of rTTc as determined by LAL method
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom。
2、 Reconstitute in a sterile aqueous buffer to an appropriate concentration。
3、 Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions。
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Storage/ Stability
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃as supplied.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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【储存条件及期限】
冻干粉于-20℃到-80℃长期保存3年，溶液状态保存期3个月，避免反复冻融。
