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Product Name
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Recombinant Rat Glial Cell Line-derived Neurotrophic Factor
(rRtGDNF)
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Synonyms
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Glial-Derived Neurotrophic Factor, ATF-1
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Source
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Expressed in E. coli.
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Molecular weight
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Approximately 29.8kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.
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Accession
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Q07731 Ser78-Ile211
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AA Sequence
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SPDKQAAALPRRERNRQAAAASPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCEAAETMYDKILKNLSRSRRLTSDKVGQACCRPVAFDDDLSFLDDSLVYHILRKHSAKRCGCI
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Purity
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＞97% by SDS-PAGE or HPLC.
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Biological activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of >5.0 × 106IU/mg
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Formulation
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Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB,150mM NaCl, 0.05 % Tween-20, pH7.4.
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Endotoxin level
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＜0.01EU/μg rRtGDNF protein as determined by LAL method.
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Reconstitution
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Before use this product, please read the direction below carefully.
1、 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
2、 Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Storage/ Stability
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For long term storage, the product should be stored ≤-20℃
36 months, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution;
3 months, -20 to -70℃ under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
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THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.
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